Rapid communicationConfocal laser endoscopy for diagnosing intraepithelial neoplasias and colorectal cancer in vivo
Section snippets
Structure and function of the confocal laser endoscope
Laser scanning confocal microscopy is an adaptation of light microscopy whereby focal laser illumination is combined with pinhole limited detection to geometrically reject out-of-focus light. In single-point scanning confocal microscopes, the point is typically scanned in a raster pattern and measurement of light returning to the detector from successive points is digitized so that an image of the scanned region can be constructed. Importantly, each resultant image is an “optical section”
Comparison of contrast agents
To evaluate the potential of contrast agents for confocal laser endoscopy, 27 patients undergoing routine surveillance screening colonoscopy at the Cabrini Hospital, Australia, between January and September 2003 were recruited for examination. The indications for screening included a personal history of polyps, tumors, or diverticular disease or a family history of colorectal cancer. In all patients examined with the prototype instrument (16 men and 11 women; median age, 63 years; range, 37–77
Discussion
In vivo confocal laser endomicroscopy is a newly developed tool that enables subsurface imaging of living cells in the mucosa during ongoing colonoscopy. The confocal laser microscope was implemented in a conventional video endoscope, which offers the possibility to combine videoendoscopy and confocal microscopy. In the present pilot trial, we report on the use of this confocal laser endoscope in humans to facilitate the prediction of colonic pathology. By directly comparing the results from in
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