p53 protein overexpression and p53 mutation analysis in patients with intestinal metaplasia of the cardia and Barrett's esophagus

Fábio Segal; Ana Paula Bohn Kaspary; João Carlos Prolla; Sandra Leistner

doi:10.1016/j.canlet.2004.01.020

p53 protein overexpression and p53 mutation analysis in patients with intestinal metaplasia of the cardia and Barrett's esophagus

Cancer Lett. 2004 Jul 16;210(2):213-8. doi: 10.1016/j.canlet.2004.01.020.

Authors

Fábio Segal¹, Ana Paula Bohn Kaspary, João Carlos Prolla, Sandra Leistner

Affiliation

¹ Medical Genetics Service, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, 90035-903, Porto Alegre, RS, Brazil.

PMID: 15183537
DOI: 10.1016/j.canlet.2004.01.020

Abstract

p53 mutation is a common genetic change in human cancers, but the clinical significance is controversial. We studied 68 patients and estimate the prevalence of intestinal metaplasia of cardia (IMC), Barrett's esophagus (BE), and p53 protein overexpression, and described molecular alterations of p53 gene exons 5 to 8. Immunohistochemical analysis showed positive p53 in 56.1-39.1% (IMC) and 60.9% (BE). Molecular analysis showed 36.6% altered cases in exon 5 and 9.8% in exon 7. In conclusion, p53 protein overexpression is common in IMC and BE. The molecular alterations observed may be due to LOH, genomic instability or other unknown alteration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aged, 80 and over
Barrett Esophagus / genetics*
Cardia / pathology*
DNA Mutational Analysis
Esophagitis / genetics*
Female
Gene Expression Profiling*
Genes, p53 / genetics*
Genomic Instability
Humans
Immunohistochemistry
Loss of Heterozygosity
Male
Metaplasia
Middle Aged
Tumor Suppressor Protein p53 / biosynthesis*
Up-Regulation

Substances

Tumor Suppressor Protein p53